Định lượng Paracetamol, Ibuprofen và Cafein trong chế phẩm bằng phép biến đổi trung bình trung tâm phổ tử ngoại tỷ đối

Lượt tải về: 7

Trương Thị Thư Hường1, Lê Công Tuấn Anh1, Đồng Thị Hà Ly2, Nguyễn Mai Hương3, Vũ Đặng Hoàng3 ,*

 1 Khoa Y Dược, trường Đại học Đà Nẵng,

 2 Khoa Dược, trường Cao đẳng Y tế Hải Phòng,

 3 Bộ môn Hóa Phân tích và Độc chất, trường Đại học Dược Hà Nội

*Tác giả liên hệ: hoangvd@hup.edu.vn

Summary

            Mean centering of UV ratio spectra in the wavelength range of 214 – 290 nm was studied for simultaneous quantification of paracetamol, ibuprofen and caffeine in pharmaceutical binary and ternary mixtures. The effect of Savitzky-Golay filter for spectral smoothing and divisor concentration was evaluated for this algorithm. The developed spectrophotometric methods could be successfully applied to the assay of paracetamol, ibuprofen and caffeine in their combined tablets and capsules, with accuracy (98-102 % recovery) and precision (RSD < 2 %). For comparison, a validated RP-HPLC method was developed by using an Agilent ZORBAX Eclipse XDB – C18 (150 × 4.6 mm; 5 µm). Isocratic elution was carried out by using the mobile phase methanol : phosphate buffer 0.01 M pH 3 (30 : 70, v/v), while the flow rate was maintained at 2 mL/min and analyses performed at 225 nm. It was shown that the mean centering – based spectrophotometric data were statistically comparable to HPLC (p > 0.05), allowing possible interchange between UV spectrophotometric and HPLC methods for routine analysis of paracetamol, ibuprofen and caffeine in their dosage forms.

Từ khóa: paracetamol, cafein, ibuprofen, trung bình trung tâm, phổ tỷ đối, sắc ký lỏng hiệu năng cao.

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